CBIO devices can be used in-line with many nucleic acid extraction kits, to improve extraction of DNA and RNA. Note: CBIO devices are not compatible with buffers that include phenol, chloroform or TRIzol. Please contact CBIO with any questions about specific applications. Using any of the OmniLyse® family of devices, it is fast and straightforward to mechanically lyse challenging gram-positive bacteria and other tough-walled organisms, including yeast and protozoan parasites, without time-consuming and less effective digestion or chemical lysis steps. Typically, unbiased lysis yields of approximately 90% can be achieved in less than one minute or approximately 99% in three minutes (up to 5 minutes for oocysts) — ideal for downstream genomic and proteomic analyses. Our patented micro-motor driven, bead-based design generates high shear force to quickly and economically disrupt hard-to-lyse organisms, including Staphylococcus, Bacillus spores, Clostridium, Mycobacteria, Candida, or Giardia/Cryptosporidium/Cyclospora oocysts, within difficult sample matrices, such as stool, soil, blood, or sputum.
OmniLyse® -inside technologies and related extraction methods are covered under one or more ot the following patents owned by Claremont BioSolutions LLC:
US 8,663,974 (issued Mar 4, 2014)
JP 5663311 (issued Dec 12, 2014)
JP 5711227 (issued Mar 13, 2015)
US 9,260,475 (issued Feb 16, 2016)
US 9,428,725 (issued Aug 30, 2016)
EP 2232259 (issued Oct 12, 2016)
JP 6040268 (issued Nov 11, 2016)
AU 2010266034 (issued Mar 30, 2017)
EP 2446047 (issued Oct 18, 2017)
US 9,873,860 (issued Jan 23, 2018)
HK 1171052 (issued Aug 3, 2018)
US D829,339 (issued on Sep 25, 2018)
US 10,428,301 (issued on Oct 1, 2019)
US 10,801,001 (issued on Oct 13, 2020)
US 11,286,447 (issued on Mar 29, 2022)
US 11,286,447 (issued on Oct 18, 2022)
US 11,479,747 (issued on Oct 25, 2022)
CA 2,711,854 (issued on Mar 21, 2023)
Additional US and international patents pending
Near-Quantitative Sampling of Genomic DNA from Various Food-Borne Eubacteria
Bead Beating-Based Continuous Flow Cell Lysis in a Microfluidic Device
Aminoglycoside-modifying Enzymes Determine the Innate Susceptibility to Aminoglycoside Antibiotics in Rapidly Growing Mycobacteria
Variability in DNA Polymerase Efficiency: Effects of Random Error, DNA Extraction Method, and Isolate Type
Solution-based Circuits Enable Rapid and Multiplexed Pathogen Detection
Proximal Bacterial Lysis and Detection in Nanoliter Wells Using Electrochemistry
Optimized Templates for Bottom-Up Growth of High-Performance Integrated Biomolecular Detectors
Ipso-Hydroxylation and Subsequent Fragmentation: a Novel Microbial Strategy To Eliminate Sulfonamide Antibiotics
Tuning the Bacterial Detection Sensitivity of Nanostructured Microelectrodes
Point of Care Diagnostics: Status and Future
Mechanical Disruption of Lysis-Resistant Bacterial Cells by Use of a Miniature, Low-Power, Disposable Device Point-of-Care Nucleic Acid Testing For Infectious Diseases
Point-of-Care Nucleic Acid Testing For Infectious Diseases
Continuous-Flow, Rapid Lysis Devices for Biodefense Nucleic Acid Diagnostic Systems
WetLab-2: Quantitative PCR Tools for Spaceflight Studies of Gene Expression aboard the International Space Station
Ch 8: Microfluidic Diagnostics for Low-resource Settings: Improving Global Health without a Power Cord
Development Status of the WetLab-2 Project: New Tools for On-orbit Real-time Quantitative Gene Expression
Low-Cost Microdevices for Point-of-Care Testing
Refining Sample Prep for Molecular DX
Use of a Disposable Lysis Device and Capture of Unamplified mycobacterial rRNA on A Liquid Bead Array for Species Determination from Liquid Cultures
Real-Time Sample Prep on the Horizon
Novel Applications for Sample Preparation: Tool Providers Launch Innovative Products to Meet Researchers' Protein-Related Demands