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 HisExpress™

Protein Protein Purification Columns

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Product Overview:
ClaremontBio's newly released HisExpress™ Protein Purification Columns can extract His-tagged proteins under native or denaturing conditions in less than five minutes.

Officially released in July of 2011, HisExpress™ Columns are quickly revolutionizing the way in which His-tagged proteins are purified. Roughly the same size as a large paper-clip and fully disposable without harming the environment, HisExpress™ Columns are ideal for laboratories that are tired of wasting time and lab space using protocols that require several instruments and reagents. Watch our live video protocol below to learn more about this exciting new technology.


Key Features:

- High quality, consistent protein extraction with purity of over 95%.

- Ultra-rapid protocol can be run in less than five minutes.

- Large binding capacity of over 1.2 mg of protein per column is 50% greater than the next leading competitor.


Applications:

Any protocol that requires His-tagged protein purification
                           
                              


Specifications:

Binding capacity of up to 1.2 mg of His-tagged proteins
Can be run under native or denaturing conditions


Data:

Figure 1:
Lysate from a pelleted cell of a 50mL bacterial culture (1 X 1011) was loaded onto a HisExpress™ Column.
Figure 2:
 Comparison of binding capacity for the HisExpress™ Column and other leading His-tagged protein purification kits.




Literature and Resources:

  HisExpress™ Protein Purification Column Simple Protocol

  HisExpress™ Protein Purification Column Native Purification Protocol

  HisExpress™ Protein Purification Column Animated Purification Protocol

  Using the OmniLyse® Cell Lysis Kit and HisExpress™ Column for Protein Purification

 

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Item# 01.365.24 HisExpress™ with OmniLyse® HL - 24 Pack Item# 01.365.48 HisExpress™ with Omnilyse® HL - 48 Pack
Pack of 24 - OmniLyse®HL/HisExpress™ Columns - Comes complete with protocol, 24 OmniLyse®HL Kits/HisExpress™ Columns, binding/elution buffers, and battery pack for complete preparation of samples from whole cells to purified His-tagged protein in under ten minutes. Pack of 48 - OmniLyse®HL/HisExpress™ Columns - Comes complete with protocol, 48 OmniLyse®HL Kits/HisExpress™ Columns, binding/elution buffers, and battery pack for complete preparation of samples from whole cells to purified His-tagged protein in under ten minutes.